Plasma albumin determination on the Cobas Bio centrifugal analyser using bromocresol green

Plasma albumin determination on the Cobas Bio centrifugal analyser using bromocresol green

Assessment of the Cobas Bio centrifugal analyser

The Cobas Bio (Roche) is a discrete spectrometric centrifugal analyser which is capable of monitoring :29 reaction cuvettes. The dispensing and analysing systems are contained within one unit and an Intel 8080A microprocessor controls the total system. Two Hamilton syringes are used to pipette samples and reagents. The sample and reagent syringes have nominal capacities of 100 and 400 #1 respec...

Immunoturbidimetric assays for serum apolipoproteins A1 and B using Cobas Bio centrifugal analyser.

Immunoturbidimetric assays for measuring the apolipoproteins A1 and B using the Cobas Bio centrifugal analyser are described. The methods were specific, offered good sensitivity (less than 0.05 g/l) and intrabatch variability, with coefficients of variation between 2.4% and 3.5%, and were cost effective. Reference ranges were calculated for a group of civil servants, aged 35 to 55 years.

Evaluation of two kinetic methods for serum amylase using a Cobas Bio centrifugal analyser

Determination of caprine serum albumin in milk using bromocresol green dye

The objective of this study was to develop and validate a colorimetric assay to measure caprine serum albumin (CSA) concentrations in milk. The principle of the say was based on the binding of albumin to bromocresol green (BCG) dye at pH 4.0. The absorption of the dye-albumin complex was determined spectrophotometrically at 640 nm. A linear relationship existed between CSA concentrations (0.01 ...